anti βactin (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
anti βactin
Anti βactin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 46609 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+%CE%B2actin/%CE%B2-Actin+Antibody/10__1038_slash_s41467___026___77609___7-622-7-9
Average 96 stars, based on 46609 article reviews
Anti βactin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 46609 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+%CE%B2actin/%CE%B2-Actin+Antibody/10__1038_slash_s41467___026___77609___7-622-7-9
Average 96 stars, based on 46609 article reviews
anti βactin - by Bioz Stars,
2026-10
96/100 stars
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SDS Page:Article Title: Onvansertib-Based Second-Line Therapies in Combination with Gemcitabine and Carboplatin in Patient-Derived Platinum-Resistant Ovarian Carcinomas Article Snippet: Snap-frozen tumor fragments were lysed in ice-cold cell extract buffer containing 50 mM TrisHCl pH 7.4, 250 mM NaCl, 0.1% Nonidet NP40, 5 mM EDTA and NaF 50 mM with a protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA). .. Protein (40 μg) was resolved on 12% SDS-PAGE gels and transferred to nitrocellulose membranes (Merck Millipore, Burlington, MA, USA), and immunoblotting was carried out with the following antibodies and visualized using Odyssey FC Imaging System (Li-COR, Lincoln, NE, USA): Article Title: Onvansertib-Based Second-Line Therapies in Combination with Gemcitabine and Carboplatin in Patient-Derived Platinum-Resistant Ovarian Carcinomas Article Snippet: Snap-frozen tumor fragments were lysed in ice-cold cell extract buffer containing 50 mM TrisHCl pH 7.4, 250 mM NaCl, 0.1% Nonidet NP40, 5 mM EDTA and NaF 50 mM with a protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA). .. Protein (40 μg) was resolved on 12% SDS-PAGE gels and transferred to nitrocellulose membranes (Merck Millipore, Burlington, MA, USA), and immunoblotting was carried out with the following antibodies and visualized using Odyssey FC Imaging System (Li-COR, Lincoln, NE, USA): Western Blot:Article Title: Onvansertib-Based Second-Line Therapies in Combination with Gemcitabine and Carboplatin in Patient-Derived Platinum-Resistant Ovarian Carcinomas Article Snippet: Snap-frozen tumor fragments were lysed in ice-cold cell extract buffer containing 50 mM TrisHCl pH 7.4, 250 mM NaCl, 0.1% Nonidet NP40, 5 mM EDTA and NaF 50 mM with a protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA). .. Protein (40 μg) was resolved on 12% SDS-PAGE gels and transferred to nitrocellulose membranes (Merck Millipore, Burlington, MA, USA), and immunoblotting was carried out with the following antibodies and visualized using Odyssey FC Imaging System (Li-COR, Lincoln, NE, USA): Article Title: STOML2 Maintains Pluripotency and Cell Cycle Integrity in Human Pluripotent Stem Cells via PHB-ERK Signaling. Article Snippet: Band intensities were quan- tified using ImageJ software, and β-actin was used as the internal loading control for normalization. .. The following primary antibodies were used for Western blotting: Article Title: Onvansertib-Based Second-Line Therapies in Combination with Gemcitabine and Carboplatin in Patient-Derived Platinum-Resistant Ovarian Carcinomas Article Snippet: Snap-frozen tumor fragments were lysed in ice-cold cell extract buffer containing 50 mM TrisHCl pH 7.4, 250 mM NaCl, 0.1% Nonidet NP40, 5 mM EDTA and NaF 50 mM with a protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA). .. Protein (40 μg) was resolved on 12% SDS-PAGE gels and transferred to nitrocellulose membranes (Merck Millipore, Burlington, MA, USA), and immunoblotting was carried out with the following antibodies and visualized using Odyssey FC Imaging System (Li-COR, Lincoln, NE, USA): Imaging:Article Title: Onvansertib-Based Second-Line Therapies in Combination with Gemcitabine and Carboplatin in Patient-Derived Platinum-Resistant Ovarian Carcinomas Article Snippet: Snap-frozen tumor fragments were lysed in ice-cold cell extract buffer containing 50 mM TrisHCl pH 7.4, 250 mM NaCl, 0.1% Nonidet NP40, 5 mM EDTA and NaF 50 mM with a protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA). .. Protein (40 μg) was resolved on 12% SDS-PAGE gels and transferred to nitrocellulose membranes (Merck Millipore, Burlington, MA, USA), and immunoblotting was carried out with the following antibodies and visualized using Odyssey FC Imaging System (Li-COR, Lincoln, NE, USA): Article Title: Onvansertib-Based Second-Line Therapies in Combination with Gemcitabine and Carboplatin in Patient-Derived Platinum-Resistant Ovarian Carcinomas Article Snippet: Snap-frozen tumor fragments were lysed in ice-cold cell extract buffer containing 50 mM TrisHCl pH 7.4, 250 mM NaCl, 0.1% Nonidet NP40, 5 mM EDTA and NaF 50 mM with a protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA). .. Protein (40 μg) was resolved on 12% SDS-PAGE gels and transferred to nitrocellulose membranes (Merck Millipore, Burlington, MA, USA), and immunoblotting was carried out with the following antibodies and visualized using Odyssey FC Imaging System (Li-COR, Lincoln, NE, USA): other:Article Title: A Combination Study of Pre- and Clinical Trial: Seaweed Consumption Reduces Aging-Associated Muscle Loss! Article Snippet: For secondary antibody reaction, each primary antibody tagged membrane was incubated at the 5% skimmed milk in 1X TBST buffer containing secondary antibody (1:10,000) either mouse (A32727, Invitrogen) or rabbit (#7074, Cell Signaling Technology) specificity at the room temperature for 1 h. An immunoblot Chemiluminescent Reagent was used to detect the proteins using a FUSION SOLO (Vilber Lourmat, Paris, France). Cell-Signaling:Article Title: Cardiac fibroblast deletion of PTBP1 modulates the profibrotic response by alternative splicing Article Snippet: .. Primary antibodies: antiαSMA, 1:500 (Sigma Aldrich, A2547); |